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Article

Stability engineering of scFvs for the development of bispecific and multivalent antibodies.

Protein engineering, design & selection : PEDS - 1 Jul 2010

Miller Brian R, Demarest Stephen J, Lugovskoy Alexey, Huang Flora, Wu Xiufeng, Snyder William B, Croner Lisa J, Wang Norman, Amatucci Aldo, Michaelson Jennifer S, Glaser Scott M

Abstract excerpt

Single-chain Fvs (scFvs) are commonly used building blocks for creating engineered diagnostic and therapeutic antibody molecules. Bispecific antibodies (BsAbs) hold particular interest due to their ability to simultaneously bind and engage two distinct targets. We describe a technology for producing stable, scalable IgG-like bispecific and multivalent antibodies based on methods for rapidly engineering thermally...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 20457695
DOI: 10.1093/protein/gzq028

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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