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Article

Increased Fab thermoresistance via VH-targeted directed evolution.

Protein engineering, design & selection : PEDS - 1 Oct 2015

Entzminger Kevin C, Johnson Jennifer L, Hyun Jeongmin, Lieberman Raquel L, Maynard Jennifer A

Abstract excerpt

Antibody aggregation is frequently mediated by the complementarity determining regions within the variable domains and can significantly decrease purification yields, shorten shelf-life and increase the risk of anti-drug immune responses. Aggregation-resistant antibodies could offset these risks; accordingly, we have developed a directed evolution strategy to improve Fab stability. A Fab-phage display vector was...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 26283664
DOI: 10.1093/protein/gzv037
PMCID: PMC4596279

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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