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Article

Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR

1 Jan 2020

Abstract excerpt

IN VITRO: affinity maturation of therapeutic monoclonal antibodies is commonly applied to achieve desired properties, such as improved binding kinetics and affinity. Currently there are no universally accepted protocols for generation of variegated antibody libraries or selection thereof. Here, we performed affinity maturation using a yeast-based single-chain variable fragment (scFv) expression system to compare...

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Share to Monoclonal and Polyclonal Antibodies Research
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PMID: 32744131
DOI: 10.1080/19420862.2020.1803646

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