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Article

Analysis of multiple ethyl methanesulfonate-mutagenized Caenorhabditis elegans strains by whole-genome sequencing.

Genetics - 1 Jun 2010

Sarin Sumeet, Bertrand Vincent, Bigelow Henry, Boyanov Alexander, Doitsidou Maria, Poole Richard J, Narula Surinder, Hobert Oliver

Abstract excerpt

Whole-genome sequencing (WGS) of organisms displaying a specific mutant phenotype is a powerful approach to identify the genetic determinants of a plethora of biological processes. We have previously validated the feasibility of this approach by identifying a point-mutated locus responsible for a specific phenotype, observed in an ethyl methanesulfonate (EMS)-mutagenized Caenorhabditis elegans strain. Here we...

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PMID: 20439776
DOI: 10.1534/genetics.110.116319
PMCID: PMC2881126

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