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Article

Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans.

Current protocols in molecular biology - 5 Jan 2017

Lehrbach Nicolas J, Ji Fei, Sadreyev Ruslan

Abstract excerpt

Forward genetic analysis using chemical mutagenesis in model organisms is a powerful tool for investigation of molecular mechanisms in biological systems. In the nematode, Caenorhabditis elegans, mutagenesis screens using ethyl methanesulfonate (EMS) have led to important insights into genetic control of animal development and physiology. A major bottleneck to this approach is identification of the causative...

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Identifiers

PMID: 28060408
DOI: 10.1002/cpmb.27
PMCID: PMC5303615

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Source receipt: pubmed, retrieved 7 Sept 2026.

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