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TADEA-PCR is a highly efficient method of amplifying unknown flanking fragments of T-DNA transformants.

Physiologia plantarum - 1 Nov 2018

Yang Yun, Cao Yuan, Xu Hua-Quan, Gao Long, Guo Xue, Liu Xue-Qiong, Zhang Li, Zhang Xue-Xin, Wang Dan-Yang

Abstract excerpt

Forward genetic analysis, widely used to find new gene functions, benefits from the availability of mutants. At present, based on Agrobacterium-mediated plant transformation technology, many transfer (T)-DNA transformants have been created. However, cloning their T-DNA insertion sites, which enables identification of the mutated genes, is still challenging. In this study, we improved adapter ligation-mediated...

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PMID: 29235671
DOI: 10.1111/ppl.12681

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

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