Article
Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning.
Proceedings of the National Academy of Sciences of the United States of America - 25 May 1999
Liu Y G, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D
Abstract excerpt
To accelerate gene isolation from plants by positional cloning, vector systems suitable for both chromosome walking and genetic complementation are highly desirable. Therefore, we developed a transformation-competent artificial chromosome (TAC) vector, pYLTAC7, that can accept and maintain large genomic DNA fragments stably in both Escherichia coli and Agrobacterium tumefaciens. Furthermore, it has the cis...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.