Article
Detection of mutations in insulin-receptor gene in NIDDM patients by analysis of single-stranded conformation polymorphisms.
Diabetes - 1 Jun 1991
O'Rahilly S, Choi W H, Patel P, Turner R C, Flier J S, Moller D E
Abstract excerpt
We used the recently described technique of single-stranded conformation-polymorphism (SSCP) analysis to examine the insulin-receptor locus. First, the ability of the method to detect known mutations and polymorphisms in the insulin-receptor coding sequence was assessed. Regions of the insulin-receptor sequence containing 16 different nucleotide changes, 9 in patient genomic DNA and 7 as cloned cDNA in plasmids,...
Topics
- Alleles
- Antisense Elements (Genetics)
- Base Sequence
- Codon
- DNA, Single-Stranded
- Diabetes Mellitus, Type 2
- Exons
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation