Article
[Development and application of non-radio isotropic PCR-SSCP analysis for genetic diagnosis].
Rinsho byori. The Japanese journal of clinical pathology - 1 Dec 1994
Kitajima I
Abstract excerpt
We applied a new and powerful technique for genetic diagnosis, so called reverse transcript-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) without using radio labeled materials (non-RI). The principle of this method is mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms. Like restriction fragment length...
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