Article
Detection of mutations in insulin receptor gene by denaturing gradient gel electrophoresis.
Diabetes - 1 Apr 1992
Barbetti F, Gejman P V, Taylor S I, Raben N, Cama A, Bonora E, Pizzo P, Moghetti P, Muggeo M, Roth J
Abstract excerpt
Denaturing gradient gel electrophoresis (DGGE) has been used to screen for mutations in the insulin receptor gene. Each of the 22 exons was amplified by the polymerase chain reaction (PCR). For each exon, one of the two PCR primers contained a guanine-cytosine (GC) clamp at its 5' end. The DNA was analyzed by electrophoresis through a polyacrylamide gel containing a gradient of denaturants. Two geometries for the...
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