Article
Detection of mutations in the human insulin gene by single strand conformation polymorphisms.
The Journal of clinical endocrinology and metabolism - 1 May 1992
Kishimoto M, Sakura H, Hayashi K, Akanuma Y, Yazaki Y, Kasuga M, Kadowaki T
Abstract excerpt
Single strand conformation polymorphisms (SSCP) is the method by which mutations can be detected in DNA amplified by the polymerase chain reaction (PCR). This method utilizes the fact that the electrophoretic mobility of single stranded DNA under nondenaturing condition depends not only on its si...
Topics
- Base Sequence
- Humans
- Insulin
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Genetic