Detection of sequence variations in the human insulin-receptor gene by parallel denaturing gradient gel electrophoresis.

doi:10.1002/humu.1380020512

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Article

Detection of sequence variations in the human insulin-receptor gene by parallel denaturing gradient gel electrophoresis.

Human mutation - 1 Jan 1993

Desbois C, Magré J, Blanquet V, Capeau J, Goossens M, Besmond C

Abstract excerpt

We developed a parallel denaturing gradient gel electrophoresis (DGGE) protocol to maximize the detection of nucleotide variants in the DNA sequence coding for the mature insulin receptor and in splice site junctions. The melting behaviours of exons 2 to 22 and flanking intronic sequences were co...

Topics

Base Sequence
DNA
Electrophoresis
Exons
Genetic Variation
Humans
Molecular Sequence Data
Nucleic Acid Denaturation
Polymorphism, Genetic
Receptor, Insulin

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