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Article

[Construction of high-quality gene mutant pool in Pichia pastoris by a PCR dependent method].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Sept 2011

Wang Rui, Yu Xiaowei, Xu Yan, Zhi Yan, Kong Yu

Abstract excerpt

We developed a method to construct a gene mutant pool in Pichia pastoris based on in vivo homologous recombination. It was an absolute PCR-dependent method (PDM) and could avoid the disadvantages of traditional mutant pool construction process such as long-experimental period, low pool capacity and inadequate abundance. The method consisted of four steps: (1) construction of recombinant expression plasmid of...

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PMID: 22117516

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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