Article
A rapid, simple, and effective method of constructing a randomly mutagenized plasmid library free from ligation.
Bioscience, biotechnology, and biochemistry - 1 Apr 2008
Koyanagi Takashi, Yoshida Erina, Minami Hiromichi, Katayama Takane, Kumagai Hidehiko
Abstract excerpt
The QuikChange site-directed mutagenesis methodology was applied to constructing a randomly mutagenized plasmid library simply by adding manganese to the reaction mixture. This method is superior to the normally employed Pol I-type polymerase-based error-prone PCR in that (i) it does not require a subsequent ligation reaction, and (ii) there is no accumulation of mutations at the same site. alpha-Complementation...
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