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Systematic screening of Escherichia coli single-gene knockout mutants for improving recombinant whole-cell biocatalysts.

Applied microbiology and biotechnology - 1 Jun 2010

Zhou Ying, Minami Takeshi, Honda Kohsuke, Omasa Takeshi, Ohtake Hisao

Abstract excerpt

Systematic screening of single-gene knockout collection of Escherichia coli BW25113 (the Keio collection) was performed to select mutants that could enhance the deethylation of 7-ethoxycoumarin catalyzed by CYP154A1. After 96-well plate high-throughput screening followed by test tube assays, four mutants (Delta cpxA, Delta gcvR, Delta glnL, and an unknown-gene-deleted one (Delta uk)) were able to increase the...

Topics

Coumarins
Cytochrome P-450 Enzyme System
Enzymes
Escherichia coli
Escherichia coli Proteins
Gene Knockout Techniques
Mutation

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 20224941
DOI: 10.1007/s00253-010-2505-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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