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Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays.

Metabolic engineering - 1 Jul 2005

Ito Mikito, Baba Tomoya, Mori Hirotada, Mori Hideo

Abstract excerpt

Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were...

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Escherichia coli
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Genes, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligonucleotide Array Sequence Analysis

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PMID: 16095938
DOI: 10.1016/j.ymben.2005.06.004

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