Article
[Construction of Escherichia coli gene knock-out mutants for engineering of fatty acid metabolism].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Jun 2013
Qu Jingqiu, Liu Cuihua, Liu Weifeng, Tao Yong, Zhu Kun
Abstract excerpt
OBJECTIVE: Gene knock out technique is very important for gene function study. We developed a simple and efficient method to knock out chromosomal genes in Escherichia coli. METHODS: Using the Escherichia coli Keio single-mutant library, we combined Red homology recombination with P1 phage transduction and developed a method to knock out the genes in Escherichia coli MG1655. RESULTS: We obtained beta-oxidation...
Topics
- Escherichia coli
- Escherichia coli Proteins
- Fatty Acids
- Gene Knockout Techniques
- Mutation