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Enhancement of recombinant enzyme activity in cpxA-deficient mutant of Escherichia coli.

Journal of bioscience and bioengineering - 1 Oct 2010

Zhou Ying, Minami Takeshi, Honda Kohsuke, Omasa Takeshi, Ohtake Hisao

Abstract excerpt

We have previously performed a systematic screening of single-gene knockout collection of Escherichia coli BW25113 (the Keio collection) to investigate the effect of cellular components on the activity of whole-cell catalysts (Zhou et al. Appl. Microbiol. Biotechnol. in press (2010)). In the present paper, the cpxA-deficient mutant of E.coli BW25113 was found to be able to enhance the activity of cytochrome...

Topics

Chromatography, High Pressure Liquid
Cytochrome P-450 Enzyme System
Escherichia coli
Escherichia coli Proteins
Mutation
Promoter Regions, Genetic
Protein Kinases
RNA, Messenger
Recombinant Proteins
Spectrophotometry, Ultraviolet

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

beta-Galactosidase

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Identifiers

PMID: 20547366
DOI: 10.1016/j.jbiosc.2010.04.013

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