Article
Genome-wide screening of the Keio collection identifies genetic determinants of Escherichia coli sensitivity and tolerance to benzoic acid.
World journal of microbiology & biotechnology - 18 Aug 2026
Öztürkel Kabakaş Hatice, Sezer Kürkçü Merve, Onat Taşdelen Kadriye Aslıhan, Di̇bek Esra, Elgi̇n Emine Sonay, Çöl Bekir
Abstract excerpt
Benzoic acid (BA) is a widely used weak-acid preservative, yet the genome-scale mechanisms underlying bacterial adaptation to preservative stress in food-associated environments remain incompletely defined. In this study, we screened the Escherichia coli Keio single-gene mutant line to identify genes associated with altered BA sensitivity or tolerance. Mutants were evaluated on LB agar containing 0.70-1.00 mg/mL...
Topics
- Benzoic Acid
- Escherichia coli
- Genome, Bacterial
- Escherichia coli Proteins
- Mutation
- Phenotype
- Adaptation, Physiological
- Microbial Sensitivity Tests
- Food Preservatives
- Drug Resistance, Bacterial