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Article

A study of energetics of cooperative interaction using a mutant lambda-repressor.

Protein engineering - 1 Sept 2000

Jana N K, Deb S, Bhattacharyya B, Mandal N C, Roy S

Abstract excerpt

A lambda-repressor mutant, S228N, which is defective in tetramer formation in the free state but retains full cooperativity, was studied in detail. Isolated single operator-bound S228N repressor shows association properties similar to those of the wild-type repressor. Fluorescence anisotropy studies with dansyl chloride-labeled repressor show a dimer-monomer dissociation constant of around 10(-5) M. The structure...

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Identifiers

PMID: 11054457
DOI: 10.1093/protein/13.9.629

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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