Article
GLUT4 is sorted to vesicles whose accumulation beneath and insertion into the plasma membrane are differentially regulated by insulin and selectively affected by insulin resistance.
Molecular biology of the cell - 15 Apr 2010
Xiong Wenyong, Jordens Ingrid, Gonzalez Eva, McGraw Timothy E
Abstract excerpt
Insulin stimulates glucose transport by recruiting the GLUT4 glucose transporter to the plasma membrane. Here we use total internal reflection fluorescence microscopy to show that two trafficking motifs of GLUT4, a FQQI motif and a TELE-based motif, target GLUT4 to specialized vesicles that accumulate adjacent to the plasma membrane of unstimulated adipocytes. Mutations of these motifs redistributed GLUT4 to...
Topics
- 3T3-L1 Cells
- Adipocytes
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Cell Membrane
- Endocytosis
- Glucose Transporter Type 4
- Humans
- Insulin
- Insulin Resistance
- Mice
- Microscopy, Fluorescence