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Article

Stromal cell independent B cell development in vitro: generation and recovery of autoreactive clones.

Journal of immunological methods - 31 Mar 2010

Holl T Matt, Haynes Barton F, Kelsoe Garnett

Abstract excerpt

We describe and characterize a stromal cell independent culture system that efficiently supports pro-B cell to IgM+ B cell development with near normal levels of IgH and Igkappa diversity. Pro-B cells present in non-adherent bone marrow cells proliferate in the presence of IL-7 and subsequent to the removal of IL-7 and addition of BAFF, differentiate normally into IgM+ B cells. B cell development in vitro closely...

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Source

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Identifiers

PMID: 20109461
DOI: 10.1016/j.jim.2010.01.007
PMCID: PMC3835306

Genes

RAG1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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