Article
Rapid and direct detection of apolipoprotein E genotypes using whole blood from humans.
Journal of toxicology and environmental health. Part A - 1 Jan 2010
Kim Suhng Wook, Heo Ji Hye, Kim Chun Huem, Yoo Dong Chul, Won Dong Hwan, Lee Seung Gwan, Cho Kyoung Jin, Song Jung Han, Park Su Jeong, Yang Young Geun, Choi Dal Woong
Abstract excerpt
Polymerase chain reaction (PCR) is a powerful molecular biological tool in the field of toxicity testing and diagnostics. The use of PCR for large-scale genetic testing requires an effective method of sample processing. Unfortunately, isolation of PCR-quality DNA is time-consuming. PCR performed directly on whole blood is preferred because of time efficiency, cost of the procedure, and possible automation for...
Topics
- Aged
- Aged, 80 and over
- Apolipoproteins E
- DNA
- Female
- Genotype
- Humans
- Male
- Middle Aged
- Nucleic Acid Amplification Techniques
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide