Article
Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation.
Clinical chemistry - 1 Jul 1991
Green E K, Bain S C, Day P J, Barnett A H, Charleson F, Jones A F, Walker M R
Abstract excerpt
A polymerase chain reaction (PCR) assay has been developed and validated by using allele-specific oligonucleotide (ASO) primers to specifically amplify E3, E2, and E4 polymorphic sequences of the human apolipoprotein E (apo E) genes. Degenerate ASOs containing one or two additional 3' mismatches...
Topics
- Alleles
- Apolipoprotein E2
- Apolipoprotein E3
- Apolipoprotein E4
- Apolipoproteins E
- Base Sequence
- DNA
- Electrophoresis, Agar Gel
- Genotype
- Humans
- Molecular Sequence Data
- Oligonucleotides
- Plasmids
- Polymerase Chain Reaction
- Reproducibility of Results
- Templates, Genetic