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Article

Directed evolution of copper nitrite reductase to a chromogenic reductant.

Protein engineering, design & selection : PEDS - 1 Mar 2010

MacPherson Iain S, Rosell Federico I, Scofield Melanie, Mauk A Grant, Murphy Michael E P

Abstract excerpt

Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput screen includes colony lift onto a nylon membrane and subsequent lysis of NiR-expressing colonies in the presence...

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Identifiers

PMID: 20083495
DOI: 10.1093/protein/gzp084
PMCID: PMC2816606

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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