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Article

Functional analysis of conserved aspartate and histidine residues located around the type 2 copper site of copper-containing nitrite reductase.

Journal of biochemistry - 1 Feb 2000

Kataoka K, Furusawa H, Takagi K, Yamaguchi K, Suzuki S

Abstract excerpt

A heterologous expression system of the blue copper-containing nitrite reductase from Alcaligenes xylosoxidans GIFU1051 (AxgNIR) was constructed, and the purified recombinant enzyme was characterized. All the characteristic spectroscopic properties and enzyme activity of native AxgNIR were retained in the copper-reconstituted recombinant protein expressed in Escherichia coli, indicating the correct coordination...

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Identifiers

PMID: 10731703
DOI: 10.1093/oxfordjournals.jbchem.a022613

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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