Article
Site-directed mutagenesis of pseudoazurin from Alcaligenes faecalis S-6; Pro80Ala mutant exhibits marked increase in reduction potential.
Protein engineering - 1 Mar 1992
Nishiyama M, Suzuki J, Ohnuki T, Chang H C, Horinouchi S, Turley S, Adman E T, Beppu T
Abstract excerpt
Pseudoazurin (a blue copper protein or cupredoxin) of a denitrifying bacterium Alcaligenes faecalis S-6 is a direct electron carrier for a Cu-containing nitrite reductase (NIR) of the same organism. Site-directed mutagenesis of the pseudoazurin was carried out using an Escherichia coli expression...
Topics
- Alcaligenes
- Azurin
- Base Sequence
- Copper
- Crystallography
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction
- Protein Denaturation
- Structure-Activity Relationship