Copper-containing nitrite reductase from Pseudomonas aureofaciens is functional in a mutationally cytochrome cd1-free background (NirS-) of Pseudomonas stutzeri.

Abstract excerpt

The structural gene, nirK, for the respiratory Cu-containing nitrite reductase from denitrifying Pseudomonas aureofaciens was isolated and sequenced. It encodes a polypeptide of 363 amino acids including a signal peptide of 24 amino acids for protein export. The sequence showed 63.8% positional i...

Topics

• Amino Acid Sequence
• Apoenzymes
• Base Sequence
• Cloning, Molecular
• Conjugation, Genetic
• Copper
• DNA, Bacterial
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Gene Library
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• Nitrite Reductases
• Nitrites
• Oxidation-Reduction
• Plasmids
• Protein Sorting Signals