Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Versatile selective evolutionary pressure using synthetic defect in universal metabolism.

Nature communications - 25 Nov 2021

Sellés Vidal Lara, Murray James W, Heap John T

Abstract excerpt

The non-natural needs of industrial applications often require new or improved enzymes. The structures and properties of enzymes are difficult to predict or design de novo. Instead, semi-rational approaches mimicking evolution entail diversification of parent enzymes followed by evaluation of isolated variants. Artificial selection pressures coupling desired enzyme properties to cell growth could overcome this...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Directed Molecular Evolution
Share to Escherichia coli
Share to Metabolic Engineering

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Metabolic Networks and Pathways

Share to Oxidoreductases

Share to Synthetic Biology

Source

Open in PubMed Open full text

Identifiers

PMID: 34824282
DOI: 10.1038/s41467-021-27266-9
PMCID: PMC8616928

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Versatile selective evolutionary pressure using synthetic defect in universal metabolism.PMID 34824282Read ↗

Selecting better biocatalysts by complementing recoded bacteriaSemantic proximity Read ↗

Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor.Semantic proximity Read ↗

Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues.Semantic proximity Read ↗

Stress-induced evolution of Escherichia coli points to original concepts in respiratory cofactor selectivity.Semantic proximity Read ↗

Engineering d-Lactate Dehydrogenase to Favor an Non-natural Cofactor Nicotinamide Cytosine Dinucleotide.Semantic proximity Read ↗

Adaptive laboratory evolution recruits the promiscuity of succinate semialdehyde dehydrogenase to repair different metabolic deficiencies.Semantic proximity Read ↗

Engineering Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase for Fully Active Amidated NAD Biosynthesis.Semantic proximity Read ↗

High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(.).Semantic proximity Read ↗

Evolution of Glucose Dehydrogenase for Cofactor Regeneration in Bioredox Processes with Denaturing Agents.Semantic proximity Read ↗

Use of adaptive laboratory evolution to discover key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal medium.Semantic proximity Read ↗

Directed evolution to re-adapt a co-evolved network within an enzyme.Semantic proximity Read ↗

Creation of bioorthogonal redox systems depending on nicotinamide flucytosine dinucleotide.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.