Article
Versatile selective evolutionary pressure using synthetic defect in universal metabolism.
Nature communications - 25 Nov 2021
Sellés Vidal Lara, Murray James W, Heap John T
Abstract excerpt
The non-natural needs of industrial applications often require new or improved enzymes. The structures and properties of enzymes are difficult to predict or design de novo. Instead, semi-rational approaches mimicking evolution entail diversification of parent enzymes followed by evaluation of isolated variants. Artificial selection pressures coupling desired enzyme properties to cell growth could overcome this...
Topics
- Directed Molecular Evolution
- Escherichia coli
- Imines
- Metabolic Engineering
- Metabolic Networks and Pathways
- Mutation
- NAD
- NADP
- Oxidoreductases
- Synthetic Biology