Article
A silent mutation made possible efficient production of active human Frk tyrosine kinase in Escherichia coli.
Bioscience, biotechnology, and biochemistry - 1 Jan 2010
Yang Xiaoyan, Kinoshita Takayoshi, Gouda Masaki, Yokota Koichi, Tada Toshiji
Abstract excerpt
Fyn-related kinase (Frk) was first identified using human breast cancer cells. It shares 51% identity with c-Src. Like all members of the Src family, Frk is thought to cause several cancers via dysregulations in signal transduction from cell-surface receptors. The excess activity of Frk on beta-cells has a crucial role in type-I diabetes. A silent mutation at Ile229 conferred a bacterial expression system on the...
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