Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

[Screening of highly active recombinant bovine enterokinase mutants mediated by green fluorescent protein and its soluble expression in Escherichia coli].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 25 Sept 2026

Han Changchun, Yue Junhao, Tu Yuwen, Chan Zhuhua, Mei Lehe, Lyu Changjiang

Abstract excerpt

Bovine enterokinase light chain (EKL), a serine protease that specifically recognizes the Asp-Asp-Asp-Asp-Lys (DDDDK) sequence, is widely used for the specific cleavage of affinity tags in recombinant proteins. However, EKL contains multiple cysteine residues and four disulfide bonds, making it difficult to fold correctly, and it tends to form inactive inclusion bodies when expressed in Escherichia coli. To...

Read the complete abstract on PubMed

Topics

Escherichia coli
Green Fluorescent Proteins
Animals
Enteropeptidase
Recombinant Proteins
Cattle

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Mutation

Solubility

Source

Identifiers

PMID: 42773660
DOI: 10.13345/j.cjb.260179

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 24 Oct 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

[Screening of highly active recombinant bovine enterokinase mutants mediated by green fluorescent protein and its soluble expression in Escherichia coli].PMID 42773660Read ↗

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 systemSemantic proximity Read ↗

Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coliSemantic proximity Read ↗

Extracellular production, purification and tag-removal of the N-terminally annexin- and ELP-tagged fluorescent proteinsSemantic proximity Read ↗

[Analysing protein folding transition state using protein engineering].Semantic proximity Read ↗

An Overview of the Parameters for Recombinant Protein Expression in Escherichia coliSemantic proximity Read ↗

A silent mutation made possible efficient production of active human Frk tyrosine kinase in Escherichia coli.Semantic proximity Read ↗

Stable mammalian serum albumins designed for bacterial expressionSemantic proximity Read ↗

[Structural study of staphylokinase related with the expression of activity].Semantic proximity Read ↗

So you want to express your protein in Escherichia coli ?Semantic proximity Read ↗

Construction of a constitutively active type III secretion system for heterologous protein secretionSemantic proximity Read ↗

An albumin‑binding domain and targeting peptide‑based recombinant protein and its enediyne‑integrated analogue exhibit directional delivery and potent inhibitory activity on pancreatic cancer with K‑ras mutation.Semantic proximity Read ↗

Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.