Article

Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host

2020-05-20

Abstract excerpt

<title>Abstract</title> <p>Background: The heterologous expression of human kinases in good purity and in a monomeric, soluble and active form can be challenging. Most of the reported successful attempts are carried out in insect cells as a host. The use of E. coli for expression is limited to a few kinases and usually is facilitated by large solubility tags that can limit biophysical studies and affect protein–p...

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Literature Corpus work: 549ceef6-0b87-5d67-8654-1bfe7bf9fb1f
DOI: 10.21203/rs.2.17140/v2

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