Article
Site-directed mutagenesis of the NH2 terminus of T4 endonuclease V. The position of the alpha NH2 moiety affects catalytic activity.
The Journal of biological chemistry - 15 Jan 1993
Schrock R D, Lloyd R S
Abstract excerpt
Reductive methylation of the alpha NH2 moiety of the DNA repair enzyme T4 endonuclease V has been shown previously to eradicate both the N-glycosylase and apyrimidinic/apurinic lyase activities of the enzyme (Schrock, R. D., III, and Lloyd, R. S. (1991) J. Biol. Chem. 266, 17631-17639). The prese...
Topics
- Amino Acid Sequence
- Bacteriophage T4
- Base Sequence
- DNA Glycosylases
- DNA Repair
- Deoxyribonuclease (Pyrimidine Dimer)
- Endodeoxyribonucleases
- Escherichia coli
- Genes, Viral
- Genotype
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- N-Glycosyl Hydrolases
- Oligodeoxyribonucleotides
- Phenotype
- Pyrimidine Dimers
- Recombinant Proteins