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Article

Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity.

Scientific reports - 16 Mar 2020

Oyama Hiroyuki, Kiguchi Yuki, Morita Izumi, Yamamoto Chika, Higashi Yuka, Taguchi Miku, Tagawa Tatsuya, Enami Yuri, Takamine Yuriko, Hasegawa Hanako, Takeuchi Atsuko, Kobayashi Norihiro

Abstract excerpt

"Antibody-breeding" has provided therapeutic/diagnostic antibody mutants with greater performance than native antibodies. Typically, random point mutations are introduced into the VH and VL domains of parent antibodies to generate diverse libraries of single-chain Fv fragments (scFvs), from which evolved mutants are selected. We produced an scFv against estradiol-17β with 11 amino acid substitutions and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32179767
DOI: 10.1038/s41598-020-61529-7
PMCID: PMC7075871

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