Article
Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection.
Journal of molecular biology - 12 Jan 1996
Schier R, Bye J, Apell G, McCall A, Adams G P, Malmqvist M, Weiner L M, Marks J D
Abstract excerpt
The use of antibodies to target tumor antigens has had limited success, partially due to the large size of IgG molecules, difficulties in constructing smaller single chain Fv (scFv) antibody fragments, and immunogenicity of murine antibodies. These limitations can be overcome by selecting human s...
Topics
- Amino Acid Sequence
- Antibody Affinity
- Bacteriophages
- Base Sequence
- Cloning, Molecular
- Gene Library
- Gene Rearrangement, B-Lymphocyte
- Genes, Immunoglobulin
- Genetic Vectors
- Humans
- Immunoglobulin Fragments
- Kinetics
- Molecular Sequence Data
- Mutation
- Receptor, ErbB-2