Article
The N-terminus of hTERT contains a DNA-binding domain and is required for telomerase activity and cellular immortalization.
Nucleic acids research - 1 Apr 2010
Sealey David C F, Zheng Le, Taboski Michael A S, Cruickshank Jennifer, Ikura Mitsuhiko, Harrington Lea A
Abstract excerpt
Telomerase defers the onset of telomere damage-induced signaling and cellular senescence by adding DNA onto chromosome ends. The ability of telomerase to elongate single-stranded telomeric DNA depends on the reverse transcriptase domain of TERT, and also relies on protein:DNA contacts outside the active site. We purified the N-terminus of human TERT (hTEN) from Escherichia coli, and found that it binds DNA with a...
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