Article
Introduction of shRNAs into primary NK cells with lentivirus.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2010
Kung Sam K P
Abstract excerpt
Natural killer (NK) cells are lymphocytes that provide an important line of defense against viruses and tumors. Technical hurdles in genetic modifications of primary NK cell ex vivo had limited our studies of protein function(s) in NK cell differentiation, acquisition of self-tolerance, and induction of anti-tumor responses. We used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient...
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