Back to search

Article

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

2018-06-14

Abstract excerpt

CRISPR/Cas9 technology is accelerating genome engineering in many cell types, but so far, gene delivery and stable gene modification have been challenging in primary NK cells. For example, transgene delivery using lentiviral or retroviral transduction resulted in a limited yield of genetically-engineered NK cells due to substantial procedure-associated NK cell apoptosis. We describe here a DNA-free method for geno...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
e5e399f8-3970-5792-909f-55b42de7abaa
DOI
10.3791/58237-v
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 RibonucleoproteinsDOI 10.3791/58237-v
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 RibonucleoproteinsSemantic proximity
A robust platform for expansion and genome editing of primary human natural killer cellsSemantic proximity
A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer ImmunotherapySemantic proximity
Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 RibonucleoproteinsSemantic proximity
Nonviral genome engineering of natural killer cellsSemantic proximity
A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer ImmunotherapySemantic proximity
Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAVSemantic proximity
Genetic Manipulation Approaches to Enhance the Clinical Application of NK Cell-Based ImmunotherapySemantic proximity
Nonviral technologies can pave the way for CAR-NK cell therapySemantic proximity
CRISPR Gene Editing of Human Primary NK and T Cells for Cancer ImmunotherapySemantic proximity
CRISPR-Targeted CAR Gene Insertion Using Cas9/RNP and AAV6 Enhances Anti-AML Activity of Primary NK CellsSemantic proximity
Non-viral approaches in CAR-NK cell engineering: connecting natural killer cell biology and gene deliverySemantic proximity
Select a neighboring publication to make it the new centre.