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Article

Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV

1 Jun 2022

Abstract excerpt

Human primary natural killer (NK) cells are being widely advanced for cancer immunotherapy. However, methods for gene editing of these cells have suffered low transduction rates, high cell death, and loss of transgene expression after expansion. Here, we developed a highly efficient method for site-specific gene insertion in NK cells using CRISPR (Cas9/RNP) and AAVs. We compared AAV vectors designed to mediate...

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PMID: 35784645
DOI: 10.1016/j.crmeth.2022.100236

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