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Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion

19 Jan 2021

Abstract excerpt

Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large numbers using a good manufacturing practice (GMP)-compliant protocol that utilizes irradiated lymphoblastoid (LCL) feeder cells. Although prestimulation of NK cells...

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PMID: 33665226
DOI: 10.1016/j.omtm.2021.01.008

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