Article
Self-splicing of a group I intron reveals partitioning of native and misfolded RNA populations in yeast.
RNA (New York, N.Y.) - 1 Dec 2006
Jackson Scott A, Koduvayur Sujatha, Woodson Sarah A
Abstract excerpt
Stable RNAs must form specific three-dimensional structures, yet many RNAs become kinetically trapped in misfolded conformations. To understand the factors that control the accuracy of RNA folding in the cell, the self-splicing activity of the Tetrahymena group I intron was compared in different genetic contexts in budding yeast. The extent of splicing was 98% when the intron was placed in its natural rDNA...
Topics
- Animals
- Base Sequence
- DNA, Ribosomal
- Genetic Complementation Test
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Polymerase II
- RNA Precursors
- RNA Splicing
- RNA, Fungal
- RNA, Ribosomal
- Saccharomyces cerevisiae
- Temperature
- Tetrahymena