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Article

An improved tetracycline-inducible expression vector for Staphylococcus aureus.

Plasmid - 1 Mar 2009

Corrigan Rebecca M, Foster Timothy J

Abstract excerpt

The tetracycline-inducible expression vector pALC2073 allowed high level expression of the cloned sasG gene but repression by uninduced cells was leaky. The -10 box of the tetR promoter was mutated to the Bacillus subtitlis consensus, which resulted in complete repression of SasG protein expression. Anhydrotetracycline at 1.28 microg ml(-1) gave the same high level of induction that was obtained with pALC2073sasG...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 18996145
DOI: 10.1016/j.plasmid.2008.10.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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