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Article

Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes.

Nucleic acids research - 12 Oct 2005

Bertram Ralph, Köstner Martin, Müller Judith, Vazquez Ramos José, Hillen Wolfgang

Abstract excerpt

We describe the construction and application of elements for random insertion of promoter containing DNA into the genome of Bacillus subtilis. The outward-facing promoter of these integrative elements termed InsTet(G+) is inducible by tetracycline so that conditional mutants are generated. We constructed three InsTet(G+) variants using different regulatory windows. In the first, the regulator gene tetR is located...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 16221969
DOI: 10.1093/nar/gni154
PMCID: PMC1253839

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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