Article
Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.
Genes & development - 1 Feb 1991
Martinez J, Georgoff I, Martinez J, Levine A J
Abstract excerpt
Primary rat embryo fibroblasts were transformed by a p53 mutant (alanine to valine change at amino acid 135) plus ras. This p53val135 mutant is temperature sensitive for a conformational change detected by the binding of a monoclonal antibody, PAb246, which recognizes the wild-type protein or the great majority of p53val135 at 32.5 degrees C. At 37 degrees C, both mutant and wild-type p53 conformational forms...
Topics
- Animals
- Antibodies, Monoclonal
- Base Sequence
- Cell Division
- Cell Line, Transformed
- Cell Transformation, Neoplastic
- Fluorescent Antibody Technique
- G1 Phase
- Genes, p53
- Molecular Sequence Data
- Mutation