Temperature-dependent switching between "wild-type" and "mutant" forms of p53-Val135.

Abstract excerpt

The p53 gene is a suppressor of abnormal cell growth but is also subject to oncogenic activation by mutation. The mutant allele p53-Val135, has recently been discovered to be temperature-sensitive and functions as an oncogene at 37 degrees C and as a tumor suppressor at 32.5 degrees C. In order to investigate the molecular mechanism underlying the temperature sensitivity of p53-Val135 rabbit reticulocyte lysate...

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, Polyomavirus Transforming
• Cell-Free System
• Erythrocytes
• Mice
• Mutation
• Phenotype
• Protein Biosynthesis
• Protein Conformation
• Rabbits
• Temperature