Article
Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus.
Yeast (Chichester, England) - 1 Jan 2010
Abdel-Banat Babiker M A, Nonklang Sanom, Hoshida Hisashi, Akada Rinji
Abstract excerpt
Kluyveromyces marxianus DMKU3-1042 is a thermotolerant yeast strain suitable for high-temperature ethanol fermentation and genetic engineering with linear DNA. We have developed a highly efficient random gene integration method with a frequency that exceeds 2.5 x 10(6) transformants/microg linear DNA, a figure comparable to what is observed with autonomously replicating plasmid transformation in Saccharomyces...
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