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Article

Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining.

Proceedings of the National Academy of Sciences of the United States of America - 17 Aug 2004

Ninomiya Yuuko, Suzuki Keiichiro, Ishii Chizu, Inoue Hirokazu

Abstract excerpt

Gene disruption and overexpression play central roles in the analysis of gene function. Homologous recombination is, in principle, the most efficient method of disrupting, modifying, or replacing a target gene. Although homologous integration of exogenous DNA into the genome occurs readily in Saccharomyces cerevisiae, it is rare in many other organisms. We identified and disrupted Neurospora crassa genes...

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Identifiers

PMID: 15299145
DOI: 10.1073/pnas.0402780101
PMCID: PMC514464

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Source receipt: pubmed, retrieved 7 Sept 2026.

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