Article
Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus.
Journal of biotechnology - 1 Aug 2007
Ribeiro Orquídea, Gombert Andreas K, Teixeira José A, Domingues Lucília
Abstract excerpt
The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant...
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