Development of a highly efficient gene targeting system induced by transient repression of YKU80 expression in Candida glabrata.

Abstract excerpt

In the pathogenic yeast Candida glabrata, gene targeting to generate knockouts and "knockins" is a potentially powerful method for the analysis of gene function. Its importance increased after the C. glabrata genome sequence project, but progress in the field is hampered by inefficient mechanisms for gene targeting. With the use of 40-bp homologous flanking DNA, no gene targeting was identified. To address this...

Topics

• Acyltransferases
• Candida glabrata
• DNA Damage
• DNA-Binding Proteins
• Fungal Proteins
• Gene Expression