Article
Two different mutants blocked in synthesis of dolichol-phosphoryl-mannose do not add glycophospholipid anchors to membrane proteins: quantitative correction of the phenotype of a CHO cell mutant with tunicamycin.
Molecular and cellular biology - 1 Jan 1991
Singh N, Tartakoff A M
Abstract excerpt
The addition of glycophospholipid (GPL) anchors to certain membrane proteins occurs in the rough endoplasmic reticulum and is essential for transport of the proteins to the plasma membrane. Limited circumstantial evidence suggests that dolichol-phosphoryl-mannose (DPM) is a donor of mannose residues of these anchors. We here report studies of a CHO cell mutant (B421) transfected to express the GPL-anchored...
Topics
- Alkaline Phosphatase
- Animals
- Antigens, Surface
- Cell Fusion
- Cell Line
- Cell Membrane
- Cricetinae
- Cricetulus
- Dolichol Monophosphate Mannose
- Genetic Complementation Test